The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible. One or more species may be used, the choice being at the discretion of the testing laboratory. At least seven fishes must be used at each test concentration and in the controls. The test substance should be administered to, at least, five concentrations in a geometric series with a factor preferably not exceeding 2.2. The limit test corresponds to one dose level of 100 mg/L. This study includes the observations of fish at least after 24, 48, 72 and 96 hours. The cumulative percentage mortality for each exposure period is plotted against concentration on logarithmic probability paper.
SANTE 2020/12830 Rev.1.
Corophium volutator chronic study
Corophium volutator acute toxicity
Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.
Arenicola marina sediment study
Mysid Acute (Static and Flow Through)
Oyster Embryo Larval Development Test
Mysid Chronic Toxicity Test
Fish: Chronic: Saltwater - Toxicity Study
Algal Acute Marine - Analytical Chemistry
Algal Acute Marine - Growth Inhibition Study
Melting Point/Melting Range
Hydrolysis as a function of pH