OECD 231

Amphibian Metamorphosis Assay

Study description if available:

This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the speciesÔøΩXenopus laevis, which is recommended for use in the Guideline. The assay uses three test chemical concentrations and the necessary controls, including a carrier control if necessary. The assay starts with tadpoles at the development stage 51 on the Nieuwkoop and Faber scale and is extended for a duration of 21 days. Four replicate test vessels are used for each treatment level and control(s). After 7 days of exposure, a sub-set of tadpoles from each treatment level is sampled for the measurement of the length of the hind-limb. At termination of 21-day exposure period, developmental stage, snout-to-vent length and hind limb length are measured on all remaining tadpoles. A sub-set of tadpoles from each treatment level is fixed (whole-body or dissected) for histopathology of the thyroid gland.

Tips: search our services here by using keywords, species, guideline number or endpoint.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
You can also filter by sector using the buttons below.

Arenicola marina sediment study

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator acute toxicity

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator chronic study

Sector:
Ecotoxicology
Read more & request a quote
ISO14669

Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.

Sector:
Ecotoxicology
Read more & request a quote

Method Development

Sector:
Analytical Chemistry
Read more & request a quote

SANTE 2020/12830 Rev.1.

Sector:
Analytical Chemistry
Read more & request a quote
OPPTS/OCSPP850.1035

Mysid Acute (Static and Flow Through)

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.1055
ISO17244

Oyster Embryo Larval Development Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.135

Mysid Chronic Toxicity Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.14

Fish: Chronic: Saltwater - Toxicity Study

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Analytical Chemistry

Sector:
Analytical Chemistry
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Growth Inhibition Study

Sector:
Ecotoxicology
Read more & request a quote
OECD 102

Melting Point/Melting Range

Sector:
Analytical Chemistry
Read more & request a quote
OECD 103

Boiling Point

Sector:
Analytical Chemistry
Read more & request a quote
OECD 104

Vapour/Pressure (isoteniscope)

Sector:
Analytical Chemistry
Read more & request a quote
OECD 105
EC A.6

Water Solubility

Sector:
Analytical Chemistry
Read more & request a quote
OECD 106

Adsorption/Desorption

Sector:
Analytical Chemistry
Read more & request a quote
OECD 107
OPPTS/OCSPP830.755
EC A.8

Partition Coefficient

Sector:
Analytical Chemistry
Read more & request a quote
OECD 109
EC A.3

Relative Density

Sector:
Analytical Chemistry
Read more & request a quote
OECD 111
OPPTS/OCSPP835.212
EC C.7

Hydrolysis as a function of pH

Sector:
Analytical Chemistry
Read more & request a quote