This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the speciesÔøΩXenopus laevis, which is recommended for use in the Guideline. The assay uses three test chemical concentrations and the necessary controls, including a carrier control if necessary. The assay starts with tadpoles at the development stage 51 on the Nieuwkoop and Faber scale and is extended for a duration of 21 days. Four replicate test vessels are used for each treatment level and control(s). After 7 days of exposure, a sub-set of tadpoles from each treatment level is sampled for the measurement of the length of the hind-limb. At termination of 21-day exposure period, developmental stage, snout-to-vent length and hind limb length are measured on all remaining tadpoles. A sub-set of tadpoles from each treatment level is fixed (whole-body or dissected) for histopathology of the thyroid gland.
SANTE 2020/12830 Rev.1.
Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.
Corophium volutator acute toxicity
Corophium volutator chronic study
Arenicola marina sediment study
Mysid Acute (Static and Flow Through)
Oyster Embryo Larval Development Test
Mysid Chronic Toxicity Test
Fish: Chronic: Saltwater - Toxicity Study
Algal Acute Marine - Analytical Chemistry
Algal Acute Marine - Growth Inhibition Study
Melting Point/Melting Range
Hydrolysis as a function of pH