OECD235

Chironomus sp Acute Immobilisation Test

Study description if available:

This Test Guideline describes an acute immobilisation assay on chronomids and is designed to complement existing Test Guidelines for chironomid chronic toxicity assays (TG 218, 219 and 233). The test method is based on TG 202: Daphnia sp. Acute Immobilisation Test. First instar Chironomus sp. larvae are exposed to a range of concentrations of the test substance in water-only vessels for a period of 48 hours. C. riparius is the preferred species but C. dilutus or C. yoshimatsui may also be used for the test. At least 20 larvae, preferably divided into four groups of five larvae each, should be used for each test concentration and for controls. In the definitive test, at least five test concentrations should be used, with a dilution water control and solvent control (if appropriate). Immobilisation is recorded at 24 and 48 hours, and if data allow, the EC50 is calculated at 24 and 48 hours. A limit test with a single concentration may also be performed at 100 mg/L of test substance or up to the practical limit of solubility (whichever is lowest) in order to demonstrate that the EC50 is greater than this concentration.

Tips: search our services here by using keywords, species, guideline number or endpoint.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
You can also filter by sector using the buttons below.
SANTE 2020/12830 Rev.1.
Read more & request a quote
ISO14669
Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.
Read more & request a quote
Arenicola marina sediment study
Read more & request a quote
Corophium volutator acute toxicity
Read more & request a quote
Corophium volutator chronic study
Read more & request a quote
OPPTS/OCSPP850.1035
Mysid Acute (Static and Flow Through)
Read more & request a quote
OPPTS/OCSPP850.1055
ISO17244
Oyster Embryo Larval Development Test
Read more & request a quote
OPPTS/OCSPP850.135
Mysid Chronic Toxicity Test
Read more & request a quote
OPPTS/OCSPP850.14
Fish Chronic: Saltwater - Toxicity Study
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253
Algal Acute Marine - Analytical Chemistry
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253
Algal Acute Marine - Growth Inhibition Study
Read more & request a quote
OECD Draft
Soil Photolysis
Read more & request a quote
OECD102
Melting Point/Melting Range
Read more & request a quote
OECD103
Boiling Point
Read more & request a quote
OECD104
Vapour/Pressure (isoteniscope)
Read more & request a quote
OECD105
EC A.6
Water Solubility
Read more & request a quote
OECD106
Adsorption/Desorption
Read more & request a quote
OECD107
OPPTS/OCSPP830.755
EC A.8
Partition Coefficient
Read more & request a quote
OECD109
EC A.3
Relative Density
Read more & request a quote