OECD 235

<i>Chironomus sp.</i> Acute Immobilisation Test

Study description:

This Test Guideline describes an acute immobilisation assay on chronomids and is designed to complement existing Test Guidelines for chironomid chronic toxicity assays (TG 218, 219 and 233). The test method is based on TG 202: Daphnia sp. Acute Immobilisation Test. First instar Chironomus sp.. larvae are exposed to a range of concentrations of the test substance in water-only vessels for a period of 48 hours. C. riparius is the preferred species but C. dilutus or C. yoshimatsui may also be used for the test. At least 20 larvae, preferably divided into four groups of five larvae each, should be used for each test concentration and for controls. In the definitive test, at least five test concentrations should be used, with a dilution water control and solvent control (if appropriate). Immobilisation is recorded at 24 and 48 hours, and if data allow, the EC50 is calculated at 24 and 48 hours. A limit test with a single concentration may also be performed at 100 mg/L of test substance or up to the practical limit of solubility (whichever is lowest) in order to demonstrate that the EC50 is greater than this concentration.

Request more information:

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Tips: search our services here by using keywords, species, guideline number or endpoint.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
You can also filter by sector using the buttons below.

SANTE 2020/12830 Rev.2.

14th February 2023.

Sector:
Analytical Chemistry
Read more & request a quote
ISO14669

Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator acute toxicity

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator chronic study

Sector:
Ecotoxicology
Read more & request a quote

Method Development

Sector:
Analytical Chemistry
Read more & request a quote

Arenicola marina sediment study

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.1035

Mysid Acute (Static and Flow Through)

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.1055
ISO17244

Oyster Embryo Larval Development Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.135

Mysid Chronic Toxicity Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.14

Fish: Chronic: Saltwater - Toxicity Study

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Analytical Chemistry

Sector:
Analytical Chemistry
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Growth Inhibition Study

Sector:
Ecotoxicology
Read more & request a quote
OECD 102

Melting Point/Melting Range

Sector:
Analytical Chemistry
Read more & request a quote
OECD 103

Boiling Point

Sector:
Analytical Chemistry
Read more & request a quote
OECD 104

Vapour/Pressure (isoteniscope)

Sector:
Analytical Chemistry
Read more & request a quote
OECD 105
EC A.6

Water Solubility

Sector:
Analytical Chemistry
Read more & request a quote
OECD 106

Adsorption/Desorption

Sector:
Analytical Chemistry
Read more & request a quote
OECD 107
OPPTS/OCSPP830.755
EC A.8

Partition Coefficient

Sector:
Analytical Chemistry
Read more & request a quote
OECD 109
EC A.3

Relative Density

Sector:
Analytical Chemistry
Read more & request a quote
OECD 111
OPPTS/OCSPP835.212
EC C.7

Hydrolysis as a function of pH

Sector:
Analytical Chemistry
Read more & request a quote