OECD 234

Fish Sexual Development Test

Study description if available:

This Test Guideline describes an assay that assesses early life-stage effects and potential adverse consequences of putative endocrine disrupting chemicals (e.g. oestrogens, androgens and steroidogenesis inhibitors) on fish sexual development. In the test, fish are exposed, from newly fertilized egg until the completion of sexual differentiation at about 60 days post hatch, to at least three concentrations of the test substance dissolved in water. In each treatment level and control(s) group(s), a minimum of four replicates is recommended. At termination of the test, two core endpoints are measured in each fish: vitellogenin concentration from head and tail or from blood sampling, and proportion of males, females, intersex and undifferentiated fish through gonadal histology. In fish species possessing a genetic sex marker, the genetic sex is identified to determine sex reversal in individual fish. The combination of the two core endocrine endpoints, vitellogenin concentration and phenotypic (and possibly genotypic) sex ratio, enable the test to indicate the mode of action of the test chemical.

Tips: search our services here by using keywords, species, guideline number or endpoint.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
You can also filter by sector using the buttons below.

Arenicola marina sediment study

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator acute toxicity

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator chronic study

Sector:
Ecotoxicology
Read more & request a quote
ISO14669

Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.

Sector:
Ecotoxicology
Read more & request a quote

Method Development

Sector:
Analytical Chemistry
Read more & request a quote

SANTE 2020/12830 Rev.1.

Sector:
Analytical Chemistry
Read more & request a quote
OPPTS/OCSPP850.1035

Mysid Acute (Static and Flow Through)

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.1055
ISO17244

Oyster Embryo Larval Development Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.135

Mysid Chronic Toxicity Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.14

Fish: Chronic: Saltwater - Toxicity Study

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Analytical Chemistry

Sector:
Analytical Chemistry
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Growth Inhibition Study

Sector:
Ecotoxicology
Read more & request a quote
OECD 102

Melting Point/Melting Range

Sector:
Analytical Chemistry
Read more & request a quote
OECD 103

Boiling Point

Sector:
Analytical Chemistry
Read more & request a quote
OECD 104

Vapour/Pressure (isoteniscope)

Sector:
Analytical Chemistry
Read more & request a quote
OECD 105
EC A.6

Water Solubility

Sector:
Analytical Chemistry
Read more & request a quote
OECD 106

Adsorption/Desorption

Sector:
Analytical Chemistry
Read more & request a quote
OECD 107
OPPTS/OCSPP830.755
EC A.8

Partition Coefficient

Sector:
Analytical Chemistry
Read more & request a quote
OECD 109
EC A.3

Relative Density

Sector:
Analytical Chemistry
Read more & request a quote
OECD 111
OPPTS/OCSPP835.212
EC C.7

Hydrolysis as a function of pH

Sector:
Analytical Chemistry
Read more & request a quote