This Test Guideline describes an in vivo screening assay for fish reproduction where sexually mature male and spawning female fish are held together and exposed to a chemical during a limited part of their life-cycle (21 days). The short term reproduction assay was validated in the fathead minnow (Pimephales promelas) and this is the recommended species. The assay is run with three test chemical concentrations and the necessary controls, including a carrier control if necessary. For the fathead minnow, four replicate test vessels are used for each treatment level and control(s). During the conduct of the assay, the egg production is measured quantitatively daily in each test vessel. At termination of the 21-day exposure period, two biomarker endpoints are measured in males and females separately, as indicators of endocrine activity of the test chemical; these endpoints are vitellogenin and secondary sexual characteristics. Gonads of both sexes are also preserved and histopathology may be evaluated to assess the reproductive fitness of the test animals and to add to the weight of evidence of other endpoints.
SANTE 2020/12830 Rev.1.
Corophium volutator chronic study
Corophium volutator acute toxicity
Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.
Arenicola marina sediment study
Mysid Acute (Static and Flow Through)
Oyster Embryo Larval Development Test
Mysid Chronic Toxicity Test
Fish: Chronic: Saltwater - Toxicity Study
Algal Acute Marine - Analytical Chemistry
Algal Acute Marine - Growth Inhibition Study
Melting Point/Melting Range
Hydrolysis as a function of pH