This Test Guideline is designed to assess effects of prolonged exposure to chemicals on the reproduction and survival of the hermaphrodite freshwater snail Lymnaea stagnalis (the Great Pond Snail). Reproducing adults of L. stagnalis are exposed to a concentration range of the test chemical and monitored for 28 days for their survival and reproduction (number of egg clutches). As additional information, the number of eggs per clutch may also be determined. Adult shell length increase may also be measured. The toxic effect of the test chemical on the cumulated number of clutches produced per individual-day is expressed as ECx by fitting an appropriate regression model to the data in order to estimate the concentration that would cause x% reduction in the reproductive output. Alternatively, the toxic effect of the test chemical can be expressed as the No Observed Effect Concentration and Lowest Observed Effect Concentration (NOEC/LOEC) values. Both ECx and NOEC/LOEC can be determined from a single study.
SANTE 2020/12830 Rev.2.
14th February 2023.
Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.
Corophium volutator acute toxicity
Corophium volutator chronic study
Arenicola marina sediment study
Mysid Acute (Static and Flow Through)
Oyster Embryo Larval Development Test
Mysid Chronic Toxicity Test
Fish: Chronic: Saltwater - Toxicity Study
Algal Acute Marine - Analytical Chemistry
Algal Acute Marine - Growth Inhibition Study
Melting Point/Melting Range
Hydrolysis as a function of pH