This test is designed to assess effects of prolonged exposure to chemicals on the reproduction and survival of the hermaphrodite freshwater snail Lymnaea stagnalis (the Great Pond Snail).

Reproducing adults of L. stagnalis are exposed to a concentration range of the testchemical and monitored for 28 days for their survival and reproduction (number of egg clutches). As additional information, the number of eggs per clutch may also be determined. Adult shell length increase may also be measured.

The toxic effect of the test chemical on the cumulated number of clutches produced per individual-day is expressed as ECx by fitting an appropriate regression model to the data in order to estimate the concentration that would cause x% reduction in the reproductive output.

Alternatively, the toxic effect of the test chemical can be expressed as the No Observed Effect Concentration and Lowest Observed Effect Concentration (NOEC/LOEC) values. Both ECx and NOEC/LOEC can be determined from a single study.

Invertebrate, chronic, freshwater.

OECD (2016), Test No. 243: Lymnaea stagnalis Reproduction Test, OECD Guidelines for the Testing of Chemicals, Section 2, OECD Publishing, Paris, https://doi.org/10.1787/9789264264335-en.