OECD 301(F)

Ready Biodegradability Study: Manometric Respirometry Test

Study description:

This Test Guideline describes six methods that permit the screening of chemicals for ready biodegradability in an aerobic aqueous medium. The methods are: the DOC Die-Away, the CO2 Evolution (Modified Sturm Test), the MITI (I) (Ministry of International Trade and Industry, Japan), the Closed Bottle, the Modified OECD Screening and the Manometric Respirometry.A solution, or suspension, of the test substance, well determined/described, in a mineral medium is inoculated and incubated under aerobic conditions in the dark or in diffuse light. The running parallel blanks with inoculum but without test substance permits to determined the endogenous activity of the inoculum. A reference compound (aniline, sodium acetate or sodium benzoate) is run in parallel to check the operation of the procedures. Normally, the test lasts for 28 days. At least two flasks or vessels containing the test substance plus inoculum, and at least two flasks or vessels containing inoculum only should be used; single vessels are sufficient for the reference compound. In general, degradation is followed by the determination of parameters such as DOC, CO2 production and oxygen uptake. The pass levels for ready biodegradability are 70% removal of DOC and 60% of ThOD or ThCO2 production for respirometric methods. These pass values have to be reached in a 10-d window within the 28-d period of the test.

Request more information:

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Tips: search our services here by using keywords, species, guideline number or endpoint.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
You can also filter by sector using the buttons below.

SANTE 2020/12830 Rev.2.

14th February 2023.

Sector:
Analytical Chemistry
Read more & request a quote
ISO14669

Crustacean Acute: Toxicity Study - Acartia Tonsa or Tisbe battaglia. Determination of acute lethal toxicity to marine copepods.

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator acute toxicity

Sector:
Ecotoxicology
Read more & request a quote

Corophium volutator chronic study

Sector:
Ecotoxicology
Read more & request a quote

Method Development

Sector:
Analytical Chemistry
Read more & request a quote

Arenicola marina sediment study

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.1035

Mysid Acute (Static and Flow Through)

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.1055
ISO17244

Oyster Embryo Larval Development Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.135

Mysid Chronic Toxicity Test

Sector:
Mixed
Read more & request a quote
OPPTS/OCSPP850.14

Fish: Chronic: Saltwater - Toxicity Study

Sector:
Ecotoxicology
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Analytical Chemistry

Sector:
Analytical Chemistry
Read more & request a quote
OPPTS/OCSPP850.45
ISO10253

Algal Acute Marine - Growth Inhibition Study

Sector:
Ecotoxicology
Read more & request a quote
OECD 102

Melting Point/Melting Range

Sector:
Analytical Chemistry
Read more & request a quote
OECD 103

Boiling Point

Sector:
Analytical Chemistry
Read more & request a quote
OECD 104

Vapour/Pressure (isoteniscope)

Sector:
Analytical Chemistry
Read more & request a quote
OECD 105
EC A.6

Water Solubility

Sector:
Analytical Chemistry
Read more & request a quote
OECD 106

Adsorption/Desorption

Sector:
Analytical Chemistry
Read more & request a quote
OECD 107
OPPTS/OCSPP830.755
EC A.8

Partition Coefficient

Sector:
Analytical Chemistry
Read more & request a quote
OECD 109
EC A.3

Relative Density

Sector:
Analytical Chemistry
Read more & request a quote
OECD 111
OPPTS/OCSPP835.212
EC C.7

Hydrolysis as a function of pH

Sector:
Analytical Chemistry
Read more & request a quote